Recombinant Human ALDOA protein (His Tag)

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- 表达系统: E.coli
- 蛋白编码: P04075
别称 |
ALDA;ALDOA;Fructose-Bisphosphate Aldolase A;Lung Cancer Antigen NY-LU-1;Muscle-Type Aldolase
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表达系统 |
E.coli
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序列 |
Asp18-Gly273
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蛋白编码 |
P04075
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种属 |
Human
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计算分子量 |
28.0 kDa
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表观分子量 |
32 kDa
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标签 |
N-His
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生物活性 |
Not validated for activity
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纯度 |
> 95% as determined by reducing SDS-PAGE.
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内毒素 |
< 10 EU/mg of the protein as determined by the LAL method
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保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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制剂 |
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
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复溶方法 |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
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背景 |
Fructose Bisphosphate Aldolase A (ALDOA) belongs to the class I fructose-bisphosphate aldolase family. ALDOA is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. In vertebrates, three forms of this ubiquitous glycolytic enzyme are found, Aldolase A in muscle, Aldolase B in liver and aldolase C in brain. Aldolase A Interacts with SNX9 and WAS. Aldolase A deficiency has been associated with myopathy and hemolytic anemia. In addition, Aldolase A plays an important role in glycolysis and gluconeogenesis, it may also act as a scaffolding protein.
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实验操作视频
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