Recombinant Human APE1/APE Protein

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- 表达系统: E.coli
- 蛋白编码: AAH02338.1
别称 |
AP Endonuclease 1;APE;APE-1REF-1;APE1;APEN;APEX;APEX Nuclease;APEX1;APX;Apurinic-Apyrimidinic Endonuclease 1;DNA-(Apurinic or Apyrimidinic Site) Lyase;HAP1;REF1;Redox Factor-1
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表达系统 |
E.coli
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序列 |
Pro2-Leu318
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蛋白编码 |
AAH02338.1
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种属 |
Human
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计算分子量 |
35.6 kDa
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表观分子量 |
40 kDa
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标签 |
None
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生物活性 |
Not validated for activity
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纯度 |
> 95 % as determined by reducing SDS-PAGE.
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内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
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保存条件 |
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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运输条件 |
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
|
制剂 |
Supplied as a 0.2 μm filtered solution of 10mM HEPES, 100mM KCl, 50% Glycerol, pH 7.4.
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复溶方法 |
Not Applicable
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背景 |
Apurinic-Apyrimidinic Endonuclease 1 (APE1) is required for efficient DNA base excision repair. When the DNA glycosylase remove the damaged bases; APE1 cleaves the AP site to allow resynthesis and ligation to complete repair. APE1 stimulates the DNA binding activity of many transcription factors; which participate in cancer promotion and progression. APE1 regulates the redox state of multiple transcription factors; such as c-Jun; c-Fos; NF-kB; p53. APEN is also involved in calcium-dependent down-regulation of PTH expression.
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Q1:重组蛋白有活性吗?可以做活性检测吗?
活性说明的见说明书bio-activity部分;如果说明书显示没有生物活性或则显示生物活性检测中,则说明这个蛋白没有生物活性数据,需要客户自己检测。
Q2:重组蛋白是否测序验证过?
在售重组蛋白默认不做测序分析/验证。
Q3:蛋白的运输条件是什么?
我们的蛋白有液体和冻干粉2种形式,一般来说冻干粉可以用冰袋、蓝冰、干冰运输;液体可以用蓝冰或干冰运输。
Q4:蛋白是通过什么方式纯化的?
一般通过纯化标签进行亲和纯化,常规有镍柱/His Tag、protein AG/Fc tag、标签抗体凝胶等。
Q5:蛋白可以用于细胞培养/注射小鼠吗?
内毒素含量小于0.1 ng/μg (1 EU/μg)的蛋白都可以用于细胞培养/注射小鼠。
Q6:蛋白发货形式可以是冻干粉吗?可以发液体吗?
默认按照说明书标注的形式发货;如果有特殊需求,需要根据蛋白情况确定。原则上,冻干粉蛋白可以发液体形式,液体蛋白不能发冻干粉形式。
Q7:蛋白Buffer里是否含有denaturants(变性剂)?
我们在售重组蛋白一般不含变性剂,具体见说明书的formulation信息。常见变性剂有:尿素和盐酸胍。
Q8:无标签蛋白纯化方式?
蛋白的纯化方式一般通过标签进行亲和纯化,无标签的蛋白是在纯化后进行了标签切除。
Q9:AF蛋白可以提供吗?
在售的重组蛋白都属于Animal component free protein。在售重组蛋白里不含任何直接来源与动物和人的制品:比如血清,血浆,全血,BSA,OVA等。
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