Recombinant Human Ataxin-3/ATXN3 protein (His Tag)
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- 表达系统： E.coli
- 蛋白编码： P54252-2
| 别称 |
ATXN3;Ataxin-3;Machado-Joseph disease protein1;Spinocerebellar ataxia type 3 protein
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| 表达系统 |
E.coli
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| 序列 |
Met1-Lys370
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| 蛋白编码 |
P54252-2
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| 种属 |
Human
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| 计算分子量 |
40.6 kDa
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| 表观分子量 |
50 kDa
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| 标签 |
N-His & C-His
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| 生物活性 |
Not validated for activity
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| 纯度 |
> 95% as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 10 EU/mg of the protein as determined by the LAL method
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| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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| 制剂 |
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
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| 复溶方法 |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
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| 背景 |
Ataxin-3 is a ubiquitously expressed nuclear protein that serves as a deubiquitinating enzyme (DUB) involved in protein homeostasis and quality control, myogenesis and degradation of misfolded chaperone substrates. As a DUB, it has two distinct features. Its N-terminal Josephin domain confers cysteine protease activity to Ataxin-3 which is important for hydrolyzing ubiquitin (Ub) linkages. The second important feature is its three Ub-interacting motifs (UIMs) through which it binds Ub conjugates and ubiquitinated proteins and bring them into proximity to trim or edit specific linkages within these Ub conjugates. It binds long poly-Ub chains and trims them. However, it has weak or no activity against chains of 4 or less Ub.
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实验操作视频
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