Recombinant Human ATG3 Protein
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- 表达系统： E.coli
- 蛋白编码： Q9NT62
| 别称 |
APG3;APG3-like;APG3L;ATG3;Autophagy-related protein 3;Protein PC3-96;Ubiquitin-like-conjugating enzyme ATG3;hApg3
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| 表达系统 |
E.coli
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| 序列 |
Met 1-Met314
|
| 蛋白编码 |
Q9NT62
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| 种属 |
Human
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| 计算分子量 |
36.0 kDa
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| 表观分子量 |
38 kDa
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| 标签 |
None
|
| 生物活性 |
Not validated for activity
|
| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
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| 保存条件 |
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
|
| 运输条件 |
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
|
| 制剂 |
Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 10% Glycerol, pH 8.0.
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| 复溶方法 |
Not Applicable
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| 背景 |
Ubiquitin-like-conjugating enzyme ATG3 (ATG3), also known as Apg3L and Apg3p, functions as a regulatory component of autophagosome biogenesis necessary for autophagy. ATG3 exhibits 98% aa sequence identity with both its mouse and rat orthologs. It is widely expressed and has highly levels in heart, skeletal muscle, kidney, liver and placenta. As an E2-like enzyme, involves in autophagy and mitochondrial homeostasis. ATG3 catalyzes the conjugation of ATG8-like proteins to PE which is essential for autophagy. ATG3 also can catalyze the conjugation of ATG12 to itself which palys a role in mitochondrial homeostasis but not in autophagy.
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实验操作视频
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