Recombinant Human BMP-2 Protein(Sumo Tag)
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- 表达系统： E.coli
- 蛋白编码： P12643
| 别称 |
BDA2;BMP-2;BMP-2A;Bmp2;BMP2;BMP2A;Bone morphogenetic protein 2;Bone morphogenetic protein 2A
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| 表达系统 |
E.coli
|
| 序列 |
Gln283-Arg396
|
| 蛋白编码 |
P12643
|
| 种属 |
Human
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| 计算分子量 |
25.4 kDa
|
| 表观分子量 |
31 kDa
|
| 标签 |
N-Sumo
|
| 生物活性 |
Not validated for activity
|
| 纯度 |
> 90% as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 10 EU/mg of the protein as determined by the LAL method
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
|
| 复溶方法 |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
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| 背景 |
Bone morphogenetic protein-2 (BMP-2) is a member of the transforming growth factor-beta (TGFB) superfamily. BMP2 is synthesized as a 60 kDa precursor that is processed in the secretory pathway to a small 18 kDa monomer, 2 monomers then associate to form the active 30 kDa homodimer,which binds to its receptor. There is also a 40-45 kDa form of BMP2,as an amino-terminal propeptide. BMP2 can induce bone formation and regeneration during early embryonic development. It is involved in the hedgehog pathway,TGF beta signaling pathway,and cytokine-cytokine receptor interaction.
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实验操作视频
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