Recombinant Human CA199(FUT3) Protein (His Tag)
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- 表达系统： E.coli
- 蛋白编码： P21217-1
| 别称 |
Lewis FT;Blood group Lewis alpha-4-fucosyltransferase;FT3B;FUT3;FucT-III;Fucosyltransferase 3;Fucosyltransferase III;Galactoside 3(4)-L-fucosyltransferase;LE
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| 表达系统 |
E.coli
|
| 序列 |
Val35-Thr361
|
| 蛋白编码 |
P21217-1
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| 种属 |
Human
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| 计算分子量 |
38.1 kDa
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| 表观分子量 |
40.9 kDa
|
| 标签 |
N-His
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| 生物活性 |
Not validated for activity
|
| 纯度 |
> 80% as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 10 EU/mg of the protein as determined by the LAL method
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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| 制剂 |
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
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| 复溶方法 |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
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| 背景 |
May catalyze alpha-1,3 and alpha-1,4 glycosidic linkages involved in the expression of Vim-2, Lewis A, Lewis B, sialyl Lewis X and Lewis X/SSEA-1 antigens. May be involved in blood group Lewis determination, Lewis-positive (Le+) individuals have an active enzyme while Lewis-negative (Le-) individuals have an inactive enzyme. Also acts on the corresponding 1,4-galactosyl derivative, forming 1,3-L-fucosyl links.
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实验操作视频
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