Recombinant Human CA9 Protein(His Tag)

Price:
- 表达系统: Mammalian
- 蛋白编码: Q16790
别称 |
CA-IX;CA9;CAH9;CAIX;Carbonate dehydratase IX;Carbonic anhydrase 9;Carbonic anhydrase IX;Carbonic dehydratase;G250;Membrane antigen MN;MN;P54/58N;pMW1;RCC associated protein G250;RCC-associated antigen G250;Renal cell carcinoma-associated antigen G250
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表达系统 |
Mammalian
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序列 |
Gln38-Asp414
|
蛋白编码 |
Q16790
|
种属 |
Human
|
计算分子量 |
41.3 kDa
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表观分子量 |
45-50 kDa
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标签 |
C-His
|
生物活性 |
Not validated for activity
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纯度 |
> 90% as determined by reducing SDS-PAGE.
|
内毒素 |
< 1.0 EU/mg of the protein as determined by the LAL method
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保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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制剂 |
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
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复溶方法 |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
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背景 |
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and is one of only two tumor-associated carbonic anhydrase isoenzymes known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12.
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实验操作视频
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