Recombinant Human CAMK1/CaMKI-alpha Protein (His Tag)
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- 表达系统： HEK293 Cells
- 蛋白编码： Q14012
| 别称 |
CAMK1;CaM Kinase I;CaM Kinase I Alpha;CaM-KI;CaMKI-Alpha;Calcium/Calmodulin-Dependent Protein Kinase Type 1
|
| 表达系统 |
HEK293 Cells
|
| 序列 |
Met1-Leu370
|
| 蛋白编码 |
Q14012
|
| 种属 |
Human
|
| 计算分子量 |
42.3 kDa
|
| 表观分子量 |
42 kDa
|
| 标签 |
C-His
|
| 生物活性 |
Not validated for activity
|
| 纯度 |
> 90 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
|
| 背景 |
Calcium/Calmodulin-Dependent Protein Kinase Type 1 (CAMK1) belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family, and CaMK subfamily. CAMK1 contains one protein kinase domain and widely expressed. CAMK1 is phosphorylated by CaMKK1 and CaMKK2 on Thr-177. CAMK1 regulates transcription activators activity, cell cycle, hormone production, cell differentiation, actin filament organization, and neurite outgrowth. CAMK1 plays a role in K+ and ANG2-mediated regulation of the aldosterone synthase (CYP11B2) to produce aldosterone in the adrenal cortex.
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实验操作视频
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