Recombinant Human CCL14a/HCC-1 Protein(Sumo Tag)
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- 表达系统： E.coli
- 蛋白编码： Q16627
| 别称 |
CCL;HCC-1/HCC;SCYA;Chemokine CC-1/CC;C-C motif chemokine 14;CC-1;CC-3;Chemokine CC-1/CC-3;HCC-1;HCC-1(1-74);HCC-1/HCC-3;HCC-3;NCC-2;Small-inducible cytokine A14;NCC2;SCYA14;CCL14;CKb1;MCIF;SCYL2
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| 表达系统 |
E.coli
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| 序列 |
Gly28-Asn93
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| 蛋白编码 |
Q16627
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| 种属 |
Human
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| 计算分子量 |
20 kDa
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| 表观分子量 |
25 kDa
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| 标签 |
N-Sumo
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| 生物活性 |
Not validated for activity
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| 纯度 |
> 90% as determined by reducing SDS-PAGE.
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| 内毒素 |
< 10 EU/mg of the protein as determined by the LAL method
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| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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| 制剂 |
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
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| 复溶方法 |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
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| 背景 |
CCL14a/HCC-1 has an important biological role in other mammals by evolving under positive selection that has been lost in Ochotonidae (subgenera Pika and Lagotona). CC chemokine ligand 14, CCL14a/HCC-1, is a human CC chemokine that is of recent interest because of its natural ability, upon proteolytic processing of the first eight NH2-terminal residues, to bind to and signal throμgh the human immunodeficiency virus type-1 (HIV-1) co-receptor, CC chemokine receptor 5 (CCR5). Embryo implantation is a complex process involving blastocyst attachment to the endometrial epithelium and subsequent trophoblast invasion of the decidua. We have previously shown that the chemokines CX3CL1 and CCL14a/HCC-1 are abundant in endometrial vasculature, epithelial, and decidual cells at this time, and that their receptors, CX3CR1 and CCR1, are present on invading human trophoblasts. CX3CL1 and CCL14a/HCC-1 promote trophoblast migration.
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实验操作视频
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