Recombinant Human CCL16/HCC-4 Protein(Trx Tag)
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- 表达系统： E.coli
- 蛋白编码： O15467
| 别称 |
HCC;Mtn;CKb;CCL;SCYA;SCYL;Monotactin;Chemokine CC;Small-Inducible Cytokine A;NCC;LCC;CCL16;CKb12;HCC-4;ILINCK;LCC-1;LEC;LMC;Mtn-1;NCC-4;NCC4;SCYA16;SCYL4;C-C motif chemokine 16;Chemokine CC-4;Chemokine LEC;IL-10-Inducible Chemokine;LCC1;Liver expressed chemokine;Liver-Expressed Chemokine;Lymphocyte and Monocyte Chemoattractant;Monotactin-1;Small-Inducible Cytokine A16;CKb12;HCC-4;LCC-1;LEC;LMC;Mtn-1;NCC-4;SCYL4;Chemokine (C-C motif) ligand 16;HCC4;IL10 inducible chemokine;Liver CC chemokine 1 precursor;Monotactin 1;Mtn1;New CC chemokine 4;Small inducible cytokine A16 precursor;Small inducible cytokine subfamily A (Cys Cys) member 16
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| 表达系统 |
E.coli
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| 序列 |
Gln24-Gln120
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| 蛋白编码 |
O15467
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| 种属 |
Human
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| 计算分子量 |
30.5 kDa
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| 表观分子量 |
31 kDa
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| 标签 |
N-Trx
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| 生物活性 |
Not validated for activity
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| 纯度 |
> 90% as determined by reducing SDS-PAGE.
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| 内毒素 |
< 10 EU/mg of the protein as determined by the LAL method
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| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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| 制剂 |
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
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| 复溶方法 |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
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| 背景 |
CCL16/HCC-4, a chemokine poorly characterized at the functional level. Human CCL16/HCC-4 is a member of the CC family, and its gene maps to human chromosome 17q. In the mouse, only a pseudogene has been identified to date. CCL16/HCC-4 is a functional ligand for CCR1, CCR2, CCR5, and CCR8. Recombinant CCL16/HCC-4 demonstrated chemotactic activity on human monocytes and lymphocytes. Based on the ability of human chemokines to exert activity on and bind to murine receptors, the TSA mouse adenocarcinoma cell line was transfected with human CCL16/HCC-4 cDNA and, in comparison with other cytokines, was shown to be the faster inducer of systemic immune response due to massive, prompt infiltration of leukocytes.
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实验操作视频
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