Recombinant Human CCL23/MPIF-1 Protein(Sumo Tag)

Price:
- 表达系统: E.coli
- 蛋白编码: P55773
别称 |
CCL;CKB;SCYA;C-C Motif Chemokine;C C motif chemokine ligand;Small-Inducible Cytokine A;Macrophage Inflammatory Protein;Myeloid Progenitor Inhibitory Factor;C C motif chemokine ligand 23;C6 beta chemokine;C-C Motif Chemokine 23;CKB-8;CK-Beta-8;Macrophage Inflammatory Protein 3;MIP-3;MPIF-1;Myeloid Progenitor Inhibitory Factor 1;Small-Inducible Cytokine A23;MIP3;MPIF1;SCYA23;CCL23;Ckb-8;MIP-3;MPIF-1;SCYA23;CCL 23;CCL23(30-99);Chemokine (C C motif) ligand 23;Chemokine CC motif ligand 23;ck beta 8;CKB 8;CKb8;Ckb-8-1;Hmrp-2a;member 23;MIP 3;MPIF 1;OTTHUMP00000163948;SCYA 23;Small inducible cytokine A 23;Small inducible cytokine subfamily A;Small inducible cytokine subfamily A (Cys-Cys)
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表达系统 |
E.coli
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序列 |
Arg22-Asn120
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蛋白编码 |
P55773
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种属 |
Human
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计算分子量 |
23.8 kDa
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表观分子量 |
30 kDa
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标签 |
N-Sumo
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生物活性 |
Not validated for activity
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纯度 |
> 90% as determined by reducing SDS-PAGE.
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内毒素 |
< 10 EU/mg of the protein as determined by the LAL method
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保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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制剂 |
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
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复溶方法 |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
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背景 |
CCL23/MPIF-1, also known as MIP 3, is a small cytokine which belongs to the CC chemokine family. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. CCL23/MPIF-1 is predominantly expressed in lung and liver tissue, but also can be detected in bone marrow and placenta. It displays chemotactic activity on resting T lymphocytes and monocytes, lower activity on neutrophils and no activity on activated T lymphocytes. CCL23/MPIF-1 is also a strong suppressor of colony formation by a multipotential hematopoietic progenitor cell line.
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Q1:重组蛋白有活性吗?可以做活性检测吗?
活性说明的见说明书bio-activity部分;如果说明书显示没有生物活性或则显示生物活性检测中,则说明这个蛋白没有生物活性数据,需要客户自己检测。
Q2:重组蛋白是否测序验证过?
在售重组蛋白默认不做测序分析/验证。
Q3:蛋白的运输条件是什么?
我们的蛋白有液体和冻干粉2种形式,一般来说冻干粉可以用冰袋、蓝冰、干冰运输;液体可以用蓝冰或干冰运输。
Q4:蛋白是通过什么方式纯化的?
一般通过纯化标签进行亲和纯化,常规有镍柱/His Tag、protein AG/Fc tag、标签抗体凝胶等。
Q5:蛋白可以用于细胞培养/注射小鼠吗?
内毒素含量小于0.1 ng/μg (1 EU/μg)的蛋白都可以用于细胞培养/注射小鼠。
Q6:蛋白发货形式可以是冻干粉吗?可以发液体吗?
默认按照说明书标注的形式发货;如果有特殊需求,需要根据蛋白情况确定。原则上,冻干粉蛋白可以发液体形式,液体蛋白不能发冻干粉形式。
Q7:蛋白Buffer里是否含有denaturants(变性剂)?
我们在售重组蛋白一般不含变性剂,具体见说明书的formulation信息。常见变性剂有:尿素和盐酸胍。
Q8:无标签蛋白纯化方式?
蛋白的纯化方式一般通过标签进行亲和纯化,无标签的蛋白是在纯化后进行了标签切除。
Q9:AF蛋白可以提供吗?
在售的重组蛋白都属于Animal component free protein。在售重组蛋白里不含任何直接来源与动物和人的制品:比如血清,血浆,全血,BSA,OVA等。
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