A130072A22Rik;C-C motif chemokine 25;CCL 25;CCL25;CCL25;Chemokine (C C motif) ligand 25;Chemokine (CC motif) ligand 25;Chemokine C C motif ligand 25;Chemokine CC motif ligand 25;Chemokine TECK;Ck beta 15;Ckb 15;Ckb15;MGC125074;MGC150327;SCY A25;SCYA 25;SCYA25;Small inducible cytokine A25;Small inducible cytokine A25 isoform 1 precursor;Small inducible cytokine A25 isoform 2 precursor;Small inducible cytokine subfamily A (Cys Cys) member 25;Small inducible cytokine subfamily A Cys Cys member 25;Small inducible cytokine subfamily A;member 25;Small-inducible cytokine A25;TECK;TECKvar;Thymus expressed chemokine;Thymus-expressed chemokine

E.coli

Gln24-Leu150

O15444

Human

26.8 kDa

34 kDa

N-Sumo

Not validated for activity

> 90% as determined by reducing SDS-PAGE.

< 10 EU/mg of the protein as determined by the LAL method

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

This product is provided as lyophilized powder which is shipped with ice packs.

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

CCL25, also known as TECK (thymus-expressed chemokine), is a CC chemokine that regulates the trafficking of lymphocytes in the thymus and small intestine. Mature mouse CCL25 shares 40% and 81% amino acid sequence identity with an human and rat CCL25, respectively . CCL25 is produced by stromal cells in the thymus and epithelial cells of the small intestine, particularly the jejunum and ileum . It binds to and induces chemoattraction through CCR9 , and both human and mouse proteins act on human CCR9 . CCR9 is expressed on immature pre-T cells and thymocytes . CCL25 induces the homing of several lymphocyte populations to the small intestine , including Integrin alpha 4 beta 7+ gamma δ T cells , Integrin alpha E beta 7+ CD8+ T cells , and IgA-producing plasma cells . In cancer, functional CCR9 mediates the metastasis of melanoma cells to the small intestine , contributes to the CCL25-dependent migration and invasion of some breast carcinomas , and attracts mesenchymal stromal cells to CCL25-expressing multiple myelomas . CCL25 contributes to the severity of chronic inflammation in rheumatoid arthritis where it attracts CCR9+ monocytes and macrophages , in endometriosis where it promotes the invasiveness of stromal cells , and in atherosclerosis where it contributes to the accumulation of CCR9+ macrophages in arterial plaques .