Recombinant Human CCL5/RANTES Protein
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- 表达系统： E.coli
- 蛋白编码： P13501
| 别称 |
C-C motif chemokine 5;D17S136E;EoCP;Eosinophil chemotactic cytokine;SCYA5;SIS-delta;Small-inducible cytokine A5;T cell-specific protein P228;T-cell-specific protein RANTES;TCP228
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| 表达系统 |
E.coli
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| 序列 |
Ser24-Ser91
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| 蛋白编码 |
P13501
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| 种属 |
Human
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| 计算分子量 |
22.3 kDa
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| 表观分子量 |
25 kDa
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| 标签 |
N-Sumo & C-His
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| 生物活性 |
Not validated for activity
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| 纯度 |
> 90% as determined by reducing SDS-PAGE.
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| 内毒素 |
< 10 EU/mg of the protein as determined by the LAL method
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| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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| 制剂 |
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
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| 复溶方法 |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
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| 背景 |
Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils
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实验操作视频
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