Recombinant Human CCL8/MCP-2 Protein
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- 表达系统： E.coli
- 蛋白编码： P80075
| 别称 |
C-C Motif Chemokine 8;CCL8;HC14;MCP-2;MCP2;Monocyte Chemoattractant Protein 2;Monocyte Chemotactic Protein 2;SCYA10;SCYA8;Small-Inducible Cytokine A8
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| 表达系统 |
E.coli
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| 序列 |
Gln24-Pro99
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| 蛋白编码 |
P80075
|
| 种属 |
Human
|
| 计算分子量 |
8.9 kDa
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| 表观分子量 |
12 kDa
|
| 标签 |
None
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| 生物活性 |
Not validated for activity
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| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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| 制剂 |
Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
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| 背景 |
Human Chemokine (C-C Motif) Ligand 8 (CCL8) is produced by human MG63 osteosarcoma cells. CCL8 shares 62% and 58% amino acid sequence identity with MCP-1 and MCP-3; respectively. All three MCP proteins are monocyte chemoattractants. CCL8 is chemotactic for and activates many different immune cells; including mast cells; eosinophils and basophils; which are implicated in allergic response; and monocytes; T cells; and NK cells that are involved in the inflammatory response. CCL8 elicits its effects by binding to several different cell surface receptors including CCR1; CCR2B and CCR5.
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实验操作视频
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