Recombinant Human CD300a/LMIR1 Protein (Fc & His Tag)
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- 表达系统： HEK293 Cells
- 蛋白编码： Q9UGN4
| 别称 |
CD300 Antigen-Like Family Member A;CD300A;CD300a;CLM-8;CMRF-35-H9;CMRF35-H;CMRF35-H9;CMRF35-Like Molecule 8;CMRF35H;IGSF12;IRC1/IRC2;IRp60;IgSF12;Immunoglobulin Superfamily Member 12;Inhibitory Receptor Protein 60;NK Inhibitory Receptor
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| 表达系统 |
HEK293 Cells
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| 序列 |
Leu18-Gln178
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| 蛋白编码 |
Q9UGN4
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| 种属 |
Human
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| 计算分子量 |
45.4 kDa
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| 表观分子量 |
60-75 kDa
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| 标签 |
C-Fc-His
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| 生物活性 |
Not validated for activity
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| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
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| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
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| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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| 制剂 |
Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 5% Trehalose, pH 8.0.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
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| 背景 |
CD300A is a single-pass type I membrane protein which belongs to the CD300 family. It contains 1 Ig-like V-type (immunoglobulin-like) domain. The CD300 family of myeloid immunoglobulin receptors includes activating (CD300b; CD300e) and inhibitory members (CD300a; CD300f); as well as molecules presenting a negative charge within their transmembrane domain (CD300c; CD300d). It is expressed not only by natural killer (NK) cells but also by T-cell subsets; B-cells; dendritic cells; mast cells; granulocytes and monocytes. CD300A is an inhibitory receptor which may contribute to the down-regulation of cytolytic activity in natural killer (NK) cells; and to the down-regulation of mast cell degranulation. CD300c is a functional immune receptor able to deliver activating signals upon ligation in RBL-2H3 mast cells.
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实验操作视频
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