Recombinant Human CD3 ε/CD3E Protein (His Tag)
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- 表达系统： HEK293 Cells
- 蛋白编码： P07766
- 应用性： ELISA
| 别称 |
CD3 epsilon;CD3E;CD3e;IMD18;T-Cell Surface Antigen T3/Leu-4 Epsilon Chain;T-Cell Surface Glycoprotein CD3 Epsilon Chain;T3E
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| 表达系统 |
HEK293 Cells
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| 序列 |
Asp23-Asp126
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| 蛋白编码 |
P07766
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| 种属 |
Human
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| 计算分子量 |
12.8 kDa
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| 表观分子量 |
15-21 kDa
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| 标签 |
C-His
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| 生物活性 |
Immobilized Human CD3E-His at 10 ug/ml (100 ul/wel)can bind Human Anti-CD3 mAb. The ED50 of Recombinant Human Anti-CD3 mAb is 3-15 ng/ml.
|
| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
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| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
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| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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| 制剂 |
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
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| 背景 |
T-Cell Surface Glycoprotein CD3 ε Chain (CD3ε) is a single-pass type I membrane protein. CD3ε contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3ε is a polypeptide encoded by the CD3E gene on chromosome 11 in humans. The T cell receptor-CD3 complex (TCR/CD3 complex) is involved in T-cell development and several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. The T cell receptor-CD3 complex is unique in having ten cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs). TCR/CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.
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实验操作视频
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