Recombinant Human CD40L/TNFSF5 Protein (His Tag)
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- 表达系统： HEK293 Cells
- 蛋白编码： P29965
| 别称 |
CD154;CD40 Ligand;CD40-L;CD40L;CD40LG;HIGM1;IGM;IMD3;T-BAM;T-Cell Antigen Gp39;TNF-Related Activation Protein;TNFSF5;TRAP;Tumor Necrosis Factor Ligand Superfamily Member 5;hCD40L
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| 表达系统 |
HEK293 Cells
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| 序列 |
Glu108-Leu261
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| 蛋白编码 |
P29965
|
| 种属 |
Human
|
| 计算分子量 |
19.1 kDa
|
| 表观分子量 |
19-22 kDa
|
| 标签 |
N-His
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| 生物活性 |
Not validated for activity
|
| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
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| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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| 制剂 |
Lyophilized from a 0.2 μm filtered solution of PBS, 1mM EDTA, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
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| 背景 |
CD40 Ligand (CD40LG) is a type II transmembrane glycoprotein that belongs to the TNF superfamily. Like other TNF superfamily members; CD40LG exists as a trimer in membrane bound and soluble form; both of which are bioactive. CD40LG is a ligand for CD40; its ligation also initiates signal transduction in CD40LG expressing cells. CD40LG is a differentiation antigen that is expressed on the surface of T-cells. It stimulates B-cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40LG has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T-cells and this is accompanied by the production of IFN-gamma; TNF-alpha; and IL2.
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实验操作视频
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