Recombinant Human CD47/IAP Protein (His Tag)
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- 表达系统： HEK293 Cells
- 蛋白编码： Q08722
- 应用性： BLI
| 别称 |
Antigenic Surface Determinant Protein OA3;CD47;IAP;Integrin-Associated Protein;Leukocyte Surface Antigen CD47;MER6;Protein MER6
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| 表达系统 |
HEK293 Cells
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| 序列 |
Gln19-Pro139
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| 蛋白编码 |
Q08722
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| 种属 |
Human
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| 计算分子量 |
14.8 kDa
|
| 表观分子量 |
30-45 kDa
|
| 标签 |
C-His
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| 生物活性 |
Loaded Anti-Human CD47 mAb-Fc on Protein A Biosensor, can bind Human CD47-His with an affinity constant of 0.22 nM as determined in BLI assay.
|
| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from a 0.2 μm filtered solution of 10mM Tris-Citrate, 150mM NaCl, pH 8.0.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
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| 背景 |
CD47(Integrin-Associated Protein;IAP) is a 40 - 60 kDa variably glycosylated atypical member of the immunoglobulin superfamily. The ubiquitously expressed CD47 binds to SIRP family members on macrophages; neutrophils; and T cells. CD47 is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The protein is also a receptor for the C-terminal cell-binding domain of thrombospondin; and it may play a role in membrane transport and signal transduction. This protein has broad tissue distribution; and is reduced in expression on Rh erythrocytes.
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实验操作视频
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