Recombinant Human CD73/NT5E Protein (His Tag)
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- 表达系统： HEK293 Cells
- 蛋白编码： AAH65937.1
- 应用性： ELISA
| 别称 |
5'-NT;5'-Nucleotidase;CALJA;CD73;E5NT;Ecto-5'-Nucleotidase;NT;NT5;NT5E;NTE;eN;eNT
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| 表达系统 |
HEK293 Cells
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| 序列 |
Trp27-Lys547
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| 蛋白编码 |
AAH65937.1
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| 种属 |
Human
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| 计算分子量 |
58.8 kDa
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| 表观分子量 |
62 kDa
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| 标签 |
C-His
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| 生物活性 |
Immobilized Human CD73-His at 2μg/ml (100 μl/well) can bind Anti-Human CD73 mAb.The ED50 of Anti-Human CD73 mAb is 3.33 ng/ml.
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| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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| 运输条件 |
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
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| 制剂 |
Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 120mM NaCl, 4mM CaCl2, 20% Glycerol, pH 7.5.
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| 复溶方法 |
Not Applicable
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| 背景 |
CD73 is a glycosyl phosphatidylinositol (GPI) anchored membrane protein that belongs to the 5'-nucleotidase family. CD73 is an ecto 5'Nucleotidase expressed by most cell types. CD73 hydrolyzes extracellular nucleotides into membrane permeable nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury. CD73 is a lymphocyte maturation marker that has functions independent of its catalytic activity. CD73 is also a regulator of leukocyte extravasation, a function that requires its 5'Nucleotidase activity. Defects in NT5E are the cause of calcification of joints and arteries (CAJA). The recombinant CD73 lacking GPI anchor is secreted as a monomer.
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实验操作视频
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