Recombinant Human CLC Protein(GST Tag)
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- 表达系统： E.coli
- 蛋白编码： Q05315
| 别称 |
LGALS;Galectin;Gal;CLC;GAL10;Gal-10;LGALS10;LGALS10A;LPPL_HUMAN;Charcot Leyden crystal protein;Eosinophil lysophospholipase;Gal 10;Galectin 10;Lysolecithin acylhydrolase;Charcot-Leyden crystal protein (CLC);Galectin-10
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| 表达系统 |
E.coli
|
| 序列 |
Ser2-Arg142
|
| 蛋白编码 |
Q05315
|
| 种属 |
Human
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| 计算分子量 |
41.5 kDa
|
| 表观分子量 |
36 kDa
|
| 标签 |
N-GST
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| 生物活性 |
Not validated for activity
|
| 纯度 |
> 95% as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 10 EU/mg of the protein as determined by the LAL method
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| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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| 制剂 |
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
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| 复溶方法 |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis
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| 背景 |
Cardiotrophin-like cytokine (CLC), also referred to as novel neurotrophin-1 (NNT-1) or B cell-stimulating factor-3 (BSF-3), is a new member of the IL-6 family of structurally related cytokines that includes IL-6, CNTF, LIF, CT-1, IL-11 and OSM. All family members share the receptor subunit gp130 that belong to the type I cytokine receptor superfamily. Ligand binding leads to gp130 homodimerization or heterodimerization (with LIF receptor or OSM receptor beta ), and induces cell signaling and functional activity. For several family members, including CNTF, IL-6, and IL-11, binding of the ligand to a specific receptor alpha subunit (CNTF R alpha, IL-6 R alpha, or IL-11 R alpha ) is required prior to gp130 homo- or hetero-dimerization.
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实验操作视频
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