Recombinant Human CX3CL1/Fractalkine Protein (His Tag)
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- 表达系统： HEK293 Cells
- 蛋白编码： P78423
| 别称 |
C-X3-C Motif Chemokine 1;CX3C Membrane-Anchored Chemokine;CX3CL1;FKN;Fractalkine;NTT;Neurotactin;SCYD1;Small-Inducible Cytokine D1
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| 表达系统 |
HEK293 Cells
|
| 序列 |
Gln25-Arg339
|
| 蛋白编码 |
P78423
|
| 种属 |
Human
|
| 计算分子量 |
34.4 kDa
|
| 表观分子量 |
50-90 kDa
|
| 标签 |
C-His
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| 生物活性 |
Not validated for activity
|
| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
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| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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| 制剂 |
Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
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| 背景 |
Human Fractalkine (CX3CL1) is a member of the CX3C family of chemokines. Human Fractalkine contains both chemokine and mucin domain. The soluble form of Fractalkine is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane bound form of Fractalkine promotes leukocytes adhesion to endothelial cells. Fractalkine regulates leukocyte adhesion and migration processes at the endothelium and binds to CX3CR1. Natural Human Fractalkine is produced as a long protein (373-amino acid). The mucin-like stalk permits it to bind to the cell surface.
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实验操作视频
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