Recombinant Human CXCL7/NAP-2 Protein
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- 表达系统： E.coli
- 蛋白编码： P02775
- 应用性： Cell culture
| 别称 |
C-X-C Motif Chemokine 7;CTAP3;CXCL7;LDGF;Leukocyte-Derived Growth Factor;MDGFSmall-Inducible Cytokine B7;Macrophage-Derived Growth Factor;PBP;PPBP;Platelet Basic Protein;SCYB7;TGB1;THBGB1
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| 表达系统 |
E.coli
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| 序列 |
Ala59-Asp128
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| 蛋白编码 |
P02775
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| 种属 |
Human
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| 计算分子量 |
8.4 kDa
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| 表观分子量 |
11 kDa
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| 标签 |
N-His
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| 生物活性 |
Measure by its ability to chemoattract BaF3 cells transfected with human CXCR2.The ED50 for this effect is <0.5 ng/mL.
|
| 纯度 |
> 98 % as determined by reducing SDS-PAGE.
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| 内毒素 |
< 0.1 EU per μg of the protein as determined by the LAL method.
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| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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| 制剂 |
Lyophilized from sterile PBS, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
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| 背景 |
Human Chemokine (C-X-C motif) Ligand 7 (CXCL7); also known as neutrophil activating peptide 2 (NAP-2); is a member of the CXC chemokines containing an ELR domain (Glu-Leu-Arg tripeptide motif). Similar to other ELR domain containing CXC chemokines; such as IL-8 and the GRO proteins; CXCL7 binds CXCR2; chemoattracts and activates neutrophils. CXCL7; Connective Tissue Activating Protein III (CTAPIII) and βthrombogulin (βTG); are proteolytically processed carboxylterminal fragments of platelet basic protein (PBP) which is found in the alphagranules of human platelets. Although CTAPIII; βTG; and PBP represent amino-terminal extended variants of NAP2 and possess the same CXC chemokine domains; these proteins do not exhibit CXCL7/NAP2 activity. CXCL7 induces cell migration through the G-protein-linked receptor CXCR-2.
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实验操作视频
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