Recombinant Human DDIT3 Protein(GST Tag)
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- 表达系统： E.coli
- 蛋白编码： P35638
| 别称 |
DDIT;GADD;DNA damage inducible transcript;DDIT3;CEBPZ;CHOP;CHOP-10;CHOP10;GADD153;C/EBPzeta;DDIT3 / CHOP;DNA damage inducible transcript 3;CEBPZ;CHOP;CHOP10;CHOP-10;DNA damage inducible transcript 3;GADD153;C/EBP homologous protein;C/EBP Homology Protein;C/EBP zeta;C/EBP-homologous protein;C/EBP-homologous protein 10;CCAAT/enhancer binding protein homologous protein;CHOP 10;DDIT 3;DDIT-3;DNA damage-inducible transcript 3 protein;GADD 153;Growth Arrest and DNA Damage Inducible Protein 153;Growth arrest and DNA damage inducible protein GADD153;Growth arrest and DNA damage-inducible protein GADD153;MGC4154
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| 表达系统 |
E.coli
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| 序列 |
Met1-Ala169
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| 蛋白编码 |
P35638
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| 种属 |
Human
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| 计算分子量 |
34.6 kDa
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| 表观分子量 |
43 kDa
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| 标签 |
N-GST
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| 生物活性 |
Not validated for activity
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| 纯度 |
> 95% as determined by reducing SDS-PAGE.
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| 内毒素 |
< 10 EU/mg of the protein as determined by the LAL method
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| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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| 制剂 |
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
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| 复溶方法 |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis
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| 背景 |
CHOP/GADD153, also known as DNA-damage-inducible transcript 3 (DDIT3), is a basic domain-leucine zipper(bZIP) transcription factor of C/EBP family. This protein has been shown to be up-regulated by several stresses, such as amino acid or glucose starvation, endoplasmic reticulum (ER) stress, osmotic stress and hypoxia. GADD153 protein may play a role in ER stress-mediated apoptosis and in disease including diabetes, brain ischemia and neurodegenerative disease. Recombinant GADD153 fused with His-tag, was expressed in E.coli and purified by conventional chromatography techniques.
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实验操作视频
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