Recombinant Human EGF Protein
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- 表达系统： E.coli
- 蛋白编码： P01133
- 应用性： Cell culture
| 别称 |
EGF;Epidermal Growth Factor;Pro-Epidermal Growth Factor;Urogastrone
|
| 表达系统 |
E.coli
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| 序列 |
Asn971-Arg1023
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| 蛋白编码 |
P01133
|
| 种属 |
Human
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| 计算分子量 |
7.2 kDa
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| 表观分子量 |
9-11 kDa
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| 标签 |
C-His
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| 生物活性 |
Measure by its ability to induce 3T3 cells proliferation. The ED50 for this effect is < 1.0 ng/mL. The specific activity of recombinant human EGF is approximately >1.0 x 106 IU/mg.
|
| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 0.1 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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| 制剂 |
Lyophilized from a 0.2 μm filtered solution of PBS, pH 8.0.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
|
| 背景 |
Epidermal growth factor (EGF) is a small 53 amino acid residue long protein that contains three disulfide bridges. It is a small mitogenic protein that is thought to be involved in mechanisms such as normal cell growth, oncogenesis, and wound healing. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. This protein shows both strong sequential and functional homology with human type-alpha transforming growth factor (hTGF alpha), which is a competitor for EGF receptor sites.
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Spectra of tryptophan fluorescence are the result of co-existence of certain most abundant stabilized excited state and certain most abundant destabilized excited state
IF: 4.098Journal： SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY (2021)PMID：33892250
实验操作视频
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