Recombinant Human ER alpha protein (His Tag)
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- 表达系统： E.coli
- 蛋白编码： P03372
| 别称 |
ER;ER-Alpha;ESR;ESR1;Estradiol Receptor;Estrogen Receptor;Estrogen Receptor α;NR3A1;Nuclear Receptor Subfamily 3 Group A Member 1
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| 表达系统 |
E.coli
|
| 序列 |
Met297-Ser554
|
| 蛋白编码 |
P03372
|
| 种属 |
Human
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| 计算分子量 |
28.3 kDa
|
| 表观分子量 |
31 kDa
|
| 标签 |
N-His
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| 生物活性 |
Not validated for activity
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| 纯度 |
> 95% as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 10 EU/mg of the protein as determined by the LAL method
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| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
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| 复溶方法 |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
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| 背景 |
Estrogen Receptor is a major ligand-activated transcription factor belonging to the nuclear hormone receptor superfamily. Estrogen Receptor is composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but they also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5' UTRs and use different promoters.
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实验操作视频
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