Recombinant Human FasL protein(His Tag)
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- 表达系统： E.coli
- 蛋白编码： P48023
- 应用性： Cell culture
| 别称 |
APT1LG1;APTL;Apo I Ligand;CD178;CD95L;TNFSF6;soluble Fas Ligand (sFasL)
|
| 表达系统 |
E.coli
|
| 序列 |
Gln 130-Leu 281
|
| 蛋白编码 |
P48023
|
| 种属 |
Human
|
| 计算分子量 |
29.5 kDa
|
| 表观分子量 |
30-35 kDa
|
| 标签 |
N-His-SUMO
|
| 生物活性 |
Measure by its ability to induce apoptosis in Jurkat cells. The ED50 for this effect is <1 ng/mL. The specific activity of recombinant human FasL is > 1 x 106 IU/mg.
|
| 纯度 |
> 98 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 0.1 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from sterile PBS,pH 8.0.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
|
| 背景 |
Fas Ligand, also known as FASLG and CD95L, is the ligand for FAS. It is a transmembrane protein which binds to TNFRSF6/FAS. Interaction of FAS with fas Ligand is critical in triggering apoptosis of some types of cells such as lymphocytes. Fas Ligand may be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both.
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实验操作视频
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