Recombinant Human FGF-17 Protein
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- 表达系统： E.coli
- 蛋白编码： O60258
- 应用性： Cell culture
| 别称 |
FGF-17;FGF17;Fibroblast Growth Factor 17
|
| 表达系统 |
E.coli
|
| 序列 |
Thr 23-Thr 216
|
| 蛋白编码 |
O60258
|
| 种属 |
Human
|
| 计算分子量 |
23.3 kDa
|
| 表观分子量 |
24 kDa
|
| 标签 |
N-His
|
| 生物活性 |
Measure by its ability to induce 3T3 cells proliferation. The ED50 for this effect is <5 ng/mL. The specific activity of recombinant human FGF-17 is > 2 x 105 IU/mg.
|
| 纯度 |
> 98 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 0.1 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from sterile PBS,pH 8.0.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
|
| 背景 |
Fibroblast Growth Factor 17 (FGF17) is a member of the heparin-binding growth factors family that is prominently expressed in the cerebellum and cortex. Proteins of this family possess broad mitogenic and cell survival activities and they are involved in a variety of biological processes including embryonic development cell growth; morphogenesis; tissue repair; tumor growth; and invasion. FGF17 plays an important role in the regulation of embryonic development and it acts as signaling molecule in the induction and patterning of the embryonic brain. In addition; FGF17 stimulates the proliferation and activation of cells that express FGF receptors.
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实验操作视频
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