Recombinant Human FGF-1/FGFa Protein
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- 表达系统： E.coli
- 蛋白编码： P05230
- 应用性： Cell culture
| 别称 |
Acidic Fibroblast Growth Factor;ECGFHeparin-Binding Growth Factor 1;Endothelial Cell Growth Factor;FGF-1;FGF1;FGFA;Fibroblast Growth Factor 1;HBGF-1;aFGF
|
| 表达系统 |
E.coli
|
| 序列 |
Phe16-Asp155
|
| 蛋白编码 |
P05230
|
| 种属 |
Human
|
| 计算分子量 |
16.8 kDa
|
| 表观分子量 |
18 kDa
|
| 标签 |
C-His
|
| 生物活性 |
Measure by its ability to induce 3T3 cells proliferation. The ED50 for this effect is <0.3 ng/mL. The specific activity of recombinant human FGF-1 is > 1 x 106 IU/mg.
|
| 纯度 |
> 98 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 0.1 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from sterile PBS,pH 8.0.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
|
| 背景 |
FGF acidic; also known as ECGF; FGF-1and HBGF-1; is a non-glycosylated heparin binding growth factor that is expressed in the brain; kidney; retina; smooth muscle cells; bone matrix; osteoblasts; astrocytes and endothelial cells. It is a mitogenic peptide that is produced by multiple cell types and stimulates the proliferation of cells of mesodermal; ectodermal; and endodermal origin. Its association with heparan sulfate is a prerequisite for activation of FGF receptors. Internalized FGF acidic migrates to the nucleus where it is phosphorylated by nuclear PKC delta; exported to the cytosol; dephosphorylated; and degraded. Intracellular FGF acidic inhibits p53 activity and proapoptotic signaling.
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实验操作视频
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