Recombinant Human FGF-2/FGFb Protein (aa 132-288)
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- 表达系统： E.coli
- 蛋白编码： P09038-4
- 应用性： Cell culture
| 别称 |
Basic Fibroblast Growth Factor;FGF-2;FGF2;FGFB;Fibroblast Growth Factor 2;HBGF-2;Heparin-Binding Growth Factor 2;bFGF
|
| 表达系统 |
E.coli
|
| 序列 |
Gly132-Ser288
|
| 蛋白编码 |
P09038-4
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| 种属 |
Human
|
| 计算分子量 |
17.4 kDa
|
| 表观分子量 |
16 kDa
|
| 标签 |
None
|
| 生物活性 |
Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED50 for this effect is 1.11 ng/ml.
|
| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 0.01 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from a 0.2 μm filtered solution of 20mM Tris, 150mM NaCl, 3% Trehalose, 4% Mannitol, pH 7.5.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
|
| 背景 |
FGF-basic is a members of the Fibroblast Growth Factors (FGFs) family.The family constitutes a large family of proteins involved in many aspects of development including cell proliferation; growth; and differentiation. They act on several cell types to regulate diverse physiologic functions including angiogenesis; cell growth; pattern formation; embryonic development; metabolic regulation; cell migration; neurotrophic effects; and tissue repair. FGF-basic is a non-glycosylated heparin binding growth factor that is expressed in the brain; pituitary; kidney; retina; bone; testis; adrenal gland liver; monocytes; epithelial cells and endothelial cells. FGF-basic signals through FGFR 1b; 1c; 2c; 3c and 4.
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实验操作视频
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