Recombinant Human Fibronectin/FN Protein
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- 表达系统： E.coli
- 蛋白编码： P02751
| 别称 |
CIG;ED-B;FINC;FN;FN1;FNZ;Fibronectin;GFND;GFND2;LETS;MSF
|
| 表达系统 |
E.coli
|
| 序列 |
Pro1270-Ser1546&Ala1721-Thr2016
|
| 蛋白编码 |
P02751
|
| 种属 |
Human
|
| 计算分子量 |
62.7 kDa
|
| 表观分子量 |
60-80 kDa
|
| 标签 |
None
|
| 生物活性 |
Not validated for activity
|
| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 0.01 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from a 0.2 μm filtered solution of 12.5 mM Citric acid, 1.25% Sucrose, 0.1% Tween 80, pH 5.5 .
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
|
| 背景 |
Fibronectin1(FN1) is a secreted protein and contains 12 fibronectin type-I domains;fibronectin type-II domains and 16 fibronectin type-III domains.Recombinant human fibronectin fragment; is a protein of ~63 kDa containing a central cell-binding domain; a high affinity heparin-binding domain II;and CS1 site within the alternatively spliced III CS region of human fibronectin. Cells bind to a VLA-4 ligand; a CS-I site; and a VLA-5 ligand; a cell attachment domain; and virus vectors binds to a heparin binding domain II; which co-locates the cell and the virus vector on NovoNectin. This process enhances the density of both cells and vectors; and facilitates the gene transduction in the result.
|
实验操作视频
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