Recombinant Human Fibronectin/FN Protein (His & Avi Tag)
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- 表达系统： E.coli
- 蛋白编码： P02751-15
| 别称 |
CIG;ED-B;FINC;FN;FN1;FNZ;Fibronectin;GFND;GFND2;LETS;MSF
|
| 表达系统 |
E.coli
|
| 序列 |
Glu1266-Thr1356
|
| 蛋白编码 |
P02751-15
|
| 种属 |
Human
|
| 计算分子量 |
13.4 kDa
|
| 表观分子量 |
15 kDa
|
| 标签 |
N-His-Avi
|
| 生物活性 |
Not validated for activity
|
| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
|
| 背景 |
Fibronectin is a high-molecular weight glycoprotein of the extracellular matrix that binds to membrane-spanning receptor proteins called integrins. Similar to integrins; fibronectin binds extracellular matrix components such as collagen; fibrin; and heparan sulfate proteoglycans. Fibronectin plays a major role in cell adhesion; growth; migration; and differentiation; and it is important for processes such as wound healing and embryonic development. Altered fibronectin expression; degradation; and organization has been associated with a number of pathologies; including cancer and fibrosis. Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer; named superfibronectin; exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth; angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.
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实验操作视频
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