Recombinant Human G-CSF protein(His Tag)
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- 表达系统： E.coli
- 蛋白编码： NP_757373
- 应用性： Cell culture
| 别称 |
CSF-3;GM-CSF beta;MGI-1G;pluripoietin
|
| 表达系统 |
E.coli
|
| 序列 |
Thr 31-Pro 204
|
| 蛋白编码 |
NP_757373
|
| 种属 |
Human
|
| 计算分子量 |
19.5 kDa
|
| 表观分子量 |
21 kDa
|
| 标签 |
N-His
|
| 生物活性 |
Measure by its ability to induce proliferation in NFS-60 cells. The ED50 for this effect is <50 pg/mL. The specific activity of recombinant human G-CSF is > 2 x 107 IU/mg.
|
| 纯度 |
> 98 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 0.1 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from sterile PBS, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
|
| 背景 |
Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
|
实验操作视频
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