Recombinant Human Grancalcin/GCA Protein (GST Tag)
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- 表达系统： E.coli
- 蛋白编码： P28676
| 别称 |
GCA;GCL;Grancalcin
|
| 表达系统 |
E.coli
|
| 序列 |
Met 1-Ile217
|
| 蛋白编码 |
P28676
|
| 种属 |
Human
|
| 计算分子量 |
50.3 kDa
|
| 表观分子量 |
50 kDa
|
| 标签 |
N-GST
|
| 生物活性 |
Not validated for activity
|
| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl，4% Sucrose，4% Mannitol，0.02% Tween 80 (w/v)，pH 8.0.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
|
| 背景 |
Grancalcin (GCA) is a member of the penta EF hand subfamily which includes sorcin; calpain and ALG2. Grancalcin is highly expressed bone marrow and also can detected in neutrophils and macrophages. Grancalcin interacts with L-plastin which known to have actin bundling activity. It indicates that Grancalcin may play an important role in the adhesion of neutrophils to fibronectin. Furthermore; Grancalcin localization is dependent upon calcium and magnesium. It associates with both the granule and membrane fractions; which suggested a role for grancalcin in granule-membrane fusion and degranulation.
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实验操作视频
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