Recombinant Human H2AX Protein(Trx Tag)
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- 表达系统： E.coli
- 蛋白编码： P16104
| 别称 |
H2A.X;H2A/X;H2AX;Histone H2AX;H2AFX;gamma H2A.X;γH2AX
|
| 表达系统 |
E.coli
|
| 序列 |
Met1-Tyr143
|
| 蛋白编码 |
P16104
|
| 种属 |
Human
|
| 计算分子量 |
35.7 kDa
|
| 表观分子量 |
35 kDa
|
| 标签 |
N-Trx
|
| 生物活性 |
Not validated for activity
|
| 纯度 |
> 90% as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 10 EU/mg of the protein as determined by the LAL method
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
|
| 复溶方法 |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis
|
| 背景 |
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. Human Histone H2AX with an N-terminal His tag expressed in E. coli and purified by conventional chromatography.
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实验操作视频
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