Recombinant Human HGF Protein (His Tag)
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- 表达系统： HEK293 Cells
- 蛋白编码： P14210
- 应用性： Cell culture
| 别称 |
HGF;HPTA;Hepatocyte growth factor;Hepatopoietin-A;SF;Scatter factor
|
| 表达系统 |
HEK293 Cells
|
| 序列 |
Gln32-Ser728
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| 蛋白编码 |
P14210
|
| 种属 |
Human
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| 计算分子量 |
26&53.7 kDa
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| 表观分子量 |
32-38&50-65 kDa
|
| 标签 |
C-His
|
| 生物活性 |
Measured by its ability to induce IL-11 secretion by Saos‑2 human osteosarcoma cells. The ED50 for this effect is 0.3-1. 5 ng/ml.
|
| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 0.01 EU per μg of the protein as determined by the LAL method.
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| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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| 制剂 |
Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 500mM NaCl, pH 8.0.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
|
| 背景 |
Hepatocyte growth factor/scatter factor (HGF/SF) is a paracrine cellular growth, motility and morphogenic factor. It belongs to the peptidase S1 family and Plasminogen subfamily, contains 4 kringle domains, 1 PAN domain and 1 peptidase S1 domain. HGF regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. HGF is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration.
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实验操作视频
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