MGC;IL1F;IL-1F;IL18;IGIF;IL-18;IL-1g;IL1F4;GIF;Iboctadekin;IFN-gamma-inducing factor;IL-1 gamma;IL-1F4;Interferon gamma-inducing factor;Interferon-gamma-inducing factor;Interleukin 18;Interleukin-1 gamma;Interleukin-18;MGC12320;IFN gamma inducing factor;IL 1 gamma;IL 18;IL 1g;IL1 gamma;IL18 protein;IL1g;IL1gamma;ILIF4;Interferon gamma inducing factor;Interleukin 1 gamma;Interleukin 18 (interferon-gamma-inducing factor);Interleukin18

E.coli

Ile116-Asp193

Q14116

Human

8.5 kDa

12 kDa

C-Avi-His

Not validated for activity

> 95% as determined by reducing SDS-PAGE.

< 10 EU/mg of the protein as determined by the LAL method

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

This product is provided as lyophilized powder which is shipped with ice packs.

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Interleukin-18 (IL-18, also known as interferon-gamma inducing factor) is a proinflammatory cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. This cytokine can induce the IFN-gamma production of T cells. The combination of IL-18 and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. IL-18 is an IL-1&minus,like cytokine that requires cleavage with caspase-1 to become active, was found to increase IgE production in a CD4+ T cells-, IL-4&minus, and STAT6&minus,dependent fashion. IL-18 and T cell receptor&minus,mediated stimulation could induce na&iuml,ve CD4+ T cells to develop into IL-4&minus,producing cells in vitro. Thus, caspase-1 and IL-18 may be critical in regulation of IgE production in vivo, providing a potential therapeutic target for allergic disorders. IL-18 production in primary synovial cultures and purified synovial fibroblasts was, in turn, upregulated by TNF-&alpha, and IL-1&beta,, suggesting that monokine expression can feed back to promote Th1 cell development in synovial membrane. Besides, synergistic combinations of IL-18, IL-12, and IL-15 may be of importance in sustaining both Th1 responses and monokine production in RA.