Recombinant Human IL18RAP/IL1R7 Protein (His Tag)

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- 表达系统: HEK293 Cells
- 蛋白编码: O95256
别称 |
IL1R;ACPL;CD218b;CDw218b;IL-18RAcP;IL18RB;IL-18Rbeta;IL-18R-beta;IL-1R7;IL-1R-7;IL-1RAcPL;IL1R7;IL18RAP; IL-1RcPL; CDw218b;IL18RβAccessory protein like;Accessory protein-like;CD218 antigen-like family member B;I18RA;IL 18RAcP;IL 1R accessory protein like;IL 1R7;IL 1RAcPL;IL-18 receptor accessory protein;IL18Rbeta;IL-1R accessory protein-like;Interleukin 18 receptor accessory protein;Interleukin-1 receptor 7;Interleukin-18 receptor accessory protein;Interleukin-18 receptor accessory protein-like;Interleukin-18 receptor beta;Interleukin-18 receptor beta chain
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表达系统 |
HEK293 Cells
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序列 |
Phe20-Arg356
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蛋白编码 |
O95256
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种属 |
Human
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计算分子量 |
39.4 kDa
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表观分子量 |
56 kDa
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标签 |
C-His
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生物活性 |
Not validated for activity
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纯度 |
> 95 % as determined by reducing SDS-PAGE.
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内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
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保存条件 |
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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运输条件 |
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
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制剂 |
Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.
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复溶方法 |
Please refer to the printed manual for detailed information.
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背景 |
IL-18RAP is a single-pass type I membrane protein and contains two Ig-like C2-type domains and one TIR domain, IL18RAP can be induced by IFN-alpha and IL12 in nature killer cells and T-cells. The coexpression of IL18R1 and IL18RAP is required for the activation of NF-kappa B and JNK in response to IL-18.Defects in IL18RAP are associated with Coeliac disease.
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Q1:重组蛋白有活性吗?可以做活性检测吗?
活性说明的见说明书bio-activity部分;如果说明书显示没有生物活性或则显示生物活性检测中,则说明这个蛋白没有生物活性数据,需要客户自己检测。
Q2:重组蛋白是否测序验证过?
在售重组蛋白默认不做测序分析/验证。
Q3:蛋白的运输条件是什么?
我们的蛋白有液体和冻干粉2种形式,一般来说冻干粉可以用冰袋、蓝冰、干冰运输;液体可以用蓝冰或干冰运输。
Q4:蛋白是通过什么方式纯化的?
一般通过纯化标签进行亲和纯化,常规有镍柱/His Tag、protein AG/Fc tag、标签抗体凝胶等。
Q5:蛋白可以用于细胞培养/注射小鼠吗?
内毒素含量小于0.1 ng/μg (1 EU/μg)的蛋白都可以用于细胞培养/注射小鼠。
Q6:蛋白发货形式可以是冻干粉吗?可以发液体吗?
默认按照说明书标注的形式发货;如果有特殊需求,需要根据蛋白情况确定。原则上,冻干粉蛋白可以发液体形式,液体蛋白不能发冻干粉形式。
Q7:蛋白Buffer里是否含有denaturants(变性剂)?
我们在售重组蛋白一般不含变性剂,具体见说明书的formulation信息。常见变性剂有:尿素和盐酸胍。
Q8:无标签蛋白纯化方式?
蛋白的纯化方式一般通过标签进行亲和纯化,无标签的蛋白是在纯化后进行了标签切除。
Q9:AF蛋白可以提供吗?
在售的重组蛋白都属于Animal component free protein。在售重组蛋白里不含任何直接来源与动物和人的制品:比如血清,血浆,全血,BSA,OVA等。
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