Recombinant Human IL2RA Protein(Fc Tag)-Elabscience伊莱瑞特【官网】

Recombinant Human IL2RA Protein(Fc Tag)

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货号：PDMH100268
说明书

说明书

Price: ¥3600.00 ¥1200.00

Size:

100 μg 20 μg

表达系统： Mammalian
蛋白编码： P01589

别称	Interleukin 2 receptor alpha chain;CD25;CD25 antigen;IDDM10;IL 2 receptor alpha subunit;IL-2 receptor subunit alpha;IL-2-RA;IL-2R subunit alpha;IL2 RA;IL2-RA;IL2R;IL2R;alpha chain;IL2RA;IL2RA;IMD41;Interleukin 2 receptor alpha;Interleukin 2 receptor;Interleukin-2 receptor subunit alpha;p55;t-cell growth factor receptor;TAC;TAC antigen;TCGFR
表达系统	Mammalian
序列	Glu22-Gln240
蛋白编码	P01589
种属	Human
计算分子量	48.9 kDa
表观分子量	60 kDa
标签	C-Fc
生物活性	Not validated for activity
纯度	> 90% as determined by reducing SDS-PAGE.
内毒素	< 1.0 EU/mg of the protein as determined by the LAL method
保存条件	Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
运输条件	This product is provided as lyophilized powder which is shipped with ice packs.
制剂	Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
复溶方法	It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
背景	The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with an the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with an interleukin 2 receptor alpha deficiency.

SDS-PAGE analysis of Human IL2RA proteins, 2 μg/lane of Recombinant Human IL2RA proteins was resolved with an SDS-PAGE under reducing conditions, showing bands at 48.9 KD

Q1:重组蛋白有活性吗？可以做活性检测吗？

活性说明的见说明书bio-activity部分；如果说明书显示没有生物活性或则显示生物活性检测中，则说明这个蛋白没有生物活性数据，需要客户自己检测。

Q2:重组蛋白是否测序验证过？

在售重组蛋白默认不做测序分析/验证。

Q3:蛋白的运输条件是什么？

我们的蛋白有液体和冻干粉2种形式，一般来说冻干粉可以用冰袋、蓝冰、干冰运输；液体可以用蓝冰或干冰运输。

Q4:蛋白是通过什么方式纯化的？

一般通过纯化标签进行亲和纯化，常规有镍柱/His Tag、protein AG/Fc tag、标签抗体凝胶等。

Q5:蛋白可以用于细胞培养/注射小鼠吗？

内毒素含量小于0.1 ng/μg (1 EU/μg)的蛋白都可以用于细胞培养/注射小鼠。

Q6:蛋白发货形式可以是冻干粉吗？可以发液体吗？

默认按照说明书标注的形式发货；如果有特殊需求，需要根据蛋白情况确定。原则上，冻干粉蛋白可以发液体形式，液体蛋白不能发冻干粉形式。