Recombinant Human IL-13 protein (SUMO,His Tag)
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- 表达系统： E.coli
- 蛋白编码： P35225
| 别称 |
BHR;IL13;IL-13;P600;ALRH;BHR1;NC30;Interleukin-13
|
| 表达系统 |
E.coli
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| 序列 |
Leu25-Asn146
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| 蛋白编码 |
P35225
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| 种属 |
Human
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| 计算分子量 |
33.3 kDa
|
| 表观分子量 |
35 kDa
|
| 标签 |
N-Sumo-His
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| 生物活性 |
Not validated for activity
|
| 纯度 |
> 95% as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 10 EU/mg of the protein as determined by the LAL method
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
|
| 复溶方法 |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
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| 背景 |
Interleukin 13 (IL-13) is a single-chain glycosylated polypeptide, which belongs to the IL-13/IL-4 family. IL-13 protein is secreted by many cell types, but especially by T helper type 2 (Th2) cells. IL-13 exerts its effects through a multi-subunit receptor comprising the alpha chain of the IL-4 receptor (IL-4R&alpha,) and at least one of two known IL-13-specific binding chains (IL-13 R&alpha,1 and IL-13 R&alpha,2). As a cytokine, IL-13 protein is critical in regulating inflammatory, immune responses and diseases. In addition, it inhibits the production of pro-inflammatory cytokines and chemokines, and thus down-regulates macrophage activity. IL-13 protein and antibody is more importantly implicated as a central mediator of immunoregulatory processes in various cell types.
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实验操作视频
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