Recombinant Human IL-32 alpha protein(His Tag)
Price:
- 表达系统： E.coli
- 蛋白编码： P24001-4
- 应用性： Cell culture
| 别称 |
TAIFa;IL-32alpha;IL-32beta;IL-32delta;IL-32gamma;TAIFb;TAIFc;TAIFd;IL32;NK4;TAIF;IL 32alpha;IL 32beta;IL 32delta;IL 32gamma;IL-32;Interleukin 32;Interleukin 32 small;Interleukin 32 theta;Interleukin-32;interleukin-32 eta;Natural killer cell transcript 4;Natural killer cells protein 4;NK 4;OTTHUMP00000236040;OTTHUMP00000236041;OTTHUMP00000236042;OTTHUMP00000236043;OTTHUMP00000236044;OTTHUMP00000236045;OTTHUMP00000236046;OTTHUMP00000236047;OTTHUMP00000236048;OTTHUMP00000236049;OTTHUMP00000236050;OTTHUMP00000236051;OTTHUMP00000236052;OTTHUMP00000236053;OTTHUMP00000236054;OTTHUMP00000241545;OTTHUMP00000241602;OTTHUMP00000241603;OTTHUMP00000241604;OTTHUMP00000241605;OTTHUMP00000241606;OTTHUMP00000241607;OTTHUMP00000241608;OTTHUMP00000241609;OTTHUMP00000241610;OTTHUMP00000241611;OTTHUMP00000241612;Tumor necrosis factor alpha-inducing factor
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| 表达系统 |
E.coli
|
| 序列 |
Met 1-Lys 131
|
| 蛋白编码 |
P24001-4
|
| 种属 |
Human
|
| 计算分子量 |
15.7 kDa
|
| 表观分子量 |
18 kDa
|
| 标签 |
C-His
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| 生物活性 |
Measure by its ability to induce TNF alpha secretion in RAW264.7 cells. The ED50 for this effect is <10 μg/mL.
|
| 纯度 |
> 98 % as determined by reducing SDS-PAGE.
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| 内毒素 |
< 0.1 EU per μg of the protein as determined by the LAL method.
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| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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| 制剂 |
Lyophilized from sterile PBS,pH 8.0.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
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| 背景 |
IL-32 is a recently discovered cytokine that induces various proinflammatory cytokines (TNF-alpha, IL-1beta, IL-6) and chemokines in both human and mouse cells through the NF-kappaB and p38 MAPK inflammatory signal pathways. It is regulated robustly by other major proinflammatory cytokines and is crucial to inflammation and immune responses. Four of the IL-32 isoforms (alpha, beta, gamma, and delta) are the most representative IL-32 transcripts, and the gamma isoform of IL-32 is the most active, although all isoforms are biologically active. IL-32, a cytokine produced mainly by T, natural killer, and epithelial cells induces significant amounts of TNFalpha and MIP-2 and increases the production of both cytokines in a dose-dependent manner. IL-32 has been implicated in inflammatory disorders, Mycobacterium tuberculosis infections, inflammatory bowel disease, and influenza A virus infection, as well as in some autoimmune diseases, such as rheumatoid arthritis, ulcerative colitis, and in the human stomach cancer, human lung cancer, and breast cancer tissues. Thus, IL-32 expression might be valuable as a biomarker for cancer.
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Q1:重组蛋白有活性吗？可以做活性检测吗？
活性说明的见说明书bio-activity部分；如果说明书显示没有生物活性或则显示生物活性检测中，则说明这个蛋白没有生物活性数据，需要客户自己检测。
Q2:重组蛋白是否测序验证过？
在售重组蛋白默认不做测序分析/验证。
Q3:蛋白的运输条件是什么？
我们的蛋白有液体和冻干粉2种形式，一般来说冻干粉可以用冰袋、蓝冰、干冰运输；液体可以用蓝冰或干冰运输。
Q4:蛋白是通过什么方式纯化的？
一般通过纯化标签进行亲和纯化，常规有镍柱/His Tag、protein AG/Fc tag、标签抗体凝胶等。
Q5:蛋白可以用于细胞培养/注射小鼠吗？
内毒素含量小于0.1 ng/μg (1 EU/μg)的蛋白都可以用于细胞培养/注射小鼠。
Q6:蛋白发货形式可以是冻干粉吗？可以发液体吗？
默认按照说明书标注的形式发货；如果有特殊需求，需要根据蛋白情况确定。原则上，冻干粉蛋白可以发液体形式，液体蛋白不能发冻干粉形式。
Q7:蛋白Buffer里是否含有denaturants（变性剂）？
我们在售重组蛋白一般不含变性剂，具体见说明书的formulation信息。常见变性剂有：尿素和盐酸胍。
Q8:无标签蛋白纯化方式？
蛋白的纯化方式一般通过标签进行亲和纯化，无标签的蛋白是在纯化后进行了标签切除。
Q9:AF蛋白可以提供吗？
在售的重组蛋白都属于Animal component free protein。在售重组蛋白里不含任何直接来源与动物和人的制品：比如血清，血浆，全血，BSA，OVA等。
实验操作视频
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