DVS;IL1F;Interleukin;C9orf;IL-1F;IL33;C9orf26;DVS27;IL1F11;NF-HEV;NFEHEV;DVS27 related protein;IL-1F11;IL-33;Interleukin-1 family member 11;Interleukin-33;Nuclear factor from high endothelial venules;NFHEV;CHROMOSOME 9 OPEN READING FRAME 26;DKFZp586H0523;IL 1F11;IL 33;Interleukin 1 family member 11;Interleukin 33;INTERLEUKIN 33 NFHEV;Interleukin 33 precursor;Interleukin33;Interleukin-33 (109-270);NF HEV;Nuclear factor for high endothelial venules;OTTHUMP00000021041;RP11 575C20.2

E.coli

His109-Thr270

O95760

Human

37.7 kDa

35 kDa

N-Trx

Not validated for activity

> 95% as determined by reducing SDS-PAGE.

< 10 EU/mg of the protein as determined by the LAL method

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

This product is provided as lyophilized powder which is shipped with ice packs.

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis

Interleukin-33 (IL-33) was initially discovered as a nuclear factor NF-HEV abundantly expressed in high endothelial venules. It is a 30-32 kD pro-inflammatory protein with intracellular and extracellular activities and a chromatin-associated cytokine of the IL-1 family with high sequence and structural similarity to IL-1 and IL-18. IL-33 is highly and selectively expressed by high endothelial venule endothelial cells (HEVECs) in human tonsils, Peyers's patches, and lymph nodes. It contains a bipartite nuclear localization signal at the C-terminus, and is targeted to the nucleus when ectopically expressed in human umbilical vein endothelial cells (HUVECs) and HeLa cells. The C-terminal fragment, corresponding to mature IL-33, binds and triggers signaling. IL-33 mediates its biological effects via Toll-interleukin 1 (IL-1) receptor (TIR) domain-containing receptor ST2, activates NF-kappaB and MAP kinases, and drives production of T(H)2-associated cytokines from in vitro polarized T(H)2 cells. In vivo, IL-33 induces the expression of IL-4, IL-5, and IL-13 and leads to severe pathological changes in mucosal organs. Human IL-33 is 270 amino acids in length.