Recombinant Human IL-34 protein(His Tag)
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- 表达系统： E.coli
- 蛋白编码： Q6ZMJ4
- 应用性： Cell culture
| 别称 |
Chromosome 16 Open Reading Frame;C16orf;Chromosome 16 Open Reading Frame 77;C16orf77;IL34
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| 表达系统 |
E.coli
|
| 序列 |
Asn 21-Pro 242
|
| 蛋白编码 |
Q6ZMJ4
|
| 种属 |
Human
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| 计算分子量 |
26.2 kDa
|
| 表观分子量 |
30 kDa
|
| 标签 |
C-His
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| 生物活性 |
Measure by its ability to induce human peripheral blood monocytes (PBMCs) proliferation. The ED50 for this effect is <30 pg/mL.
|
| 纯度 |
> 98 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 0.1 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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| 制剂 |
Lyophilized from sterile PBS,pH 8.0.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
|
| 背景 |
Interleukin-34 (IL-34) is a protein that promotes the proliferation,survival and differentiation of monocytes and macrophages. It also promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. IL-34 plays an important role in the regulation of osteoclast proliferation and differentiation,and in the regulation of bone resorption. Signaling via CSF1R and its downstream effectors stimulates phosphorylation of MAPK1/ERK2 AND MAPK3/ERK1.
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实验操作视频
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