Recombinant Human IL-6/Interleukin-6 Protein
Price:
- 表达系统： E.coli
- 蛋白编码： P05231
- 应用性： Cell culture
| 别称 |
IFNB;Interleukin HP;Interferon Beta;B-Cell Stimulatory Factor;IFN-Beta;B-cell hybridoma growth factor;B-Cell Stimulatory Factor 2;BSF2;BSF-2;CDF;CTL Differentiation Factor;HGF;HSF;Hybridoma Growth Factor;IFN-Beta-2;IL-6;Interferon Beta-2;Interleukin 6;Interleukin HP-1;Interleukin-6;IL6;IFNB2;B cell differentiation factor;B cell stimulatory factor 2;BSF 2;Hepatocyte stimulatory factor;Hybridoma growth factor Interferon beta-2;IL 6;Interferon beta 2;Interleukin 6 (interferon beta 2);Interleukin BSF 2;Interleukin-6;IL-6;B-cell hybridoma growth factor;Interleukin HP-1
|
| 表达系统 |
E.coli
|
| 序列 |
Val30-Met212
|
| 蛋白编码 |
P05231
|
| 种属 |
Human
|
| 计算分子量 |
21.8 kDa
|
| 表观分子量 |
22 kDa
|
| 标签 |
C-His
|
| 生物活性 |
1. Measure by its ability to induce proliferation in TF-1 cells. The ED50 for this effect is <0.5 ng/mL. The specific activity of recombinant human IL-6 is approximately >5 x 108 IU/mg.
2. Measure by its ability to induce proliferation in MCF-7 cells. The ED50 for this effect is <4.4 ng/mL. |
| 纯度 |
> 98 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 0.1 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from sterile PBS,pH 8.0.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
|
| 背景 |
Cytokines of the IL6/GCSF/MGF family are glycoproteins of about 170 to 180 amino acid residues that contain four conserved cysteine residues involved in two disulfide bonds. They have a compact, globular fold (similar to other interleukins), stabilized by the 2 disulfide bonds. One half of the structure is dominated by a 4 alpha-helix bundle with a left-handed twist; the helices are anti-parallel, with 2 overhand connections, which fall into a 2-stranded anti-parallel beta-sheet. The fourth alpha helix is important to the biological activity of the molecule. Interleukin-6 (IL-6) is an important proinflammatory and immunoregulatory cytokine expressed by various cells. Interleukin-6 has been shown to inhibit the growth of early stage and to promote the proliferation of advanced stage melanoma cells in vitro.
|
Q1:重组蛋白有活性吗？可以做活性检测吗？
活性说明的见说明书bio-activity部分；如果说明书显示没有生物活性或则显示生物活性检测中，则说明这个蛋白没有生物活性数据，需要客户自己检测。
Q2:重组蛋白是否测序验证过？
在售重组蛋白默认不做测序分析/验证。
Q3:蛋白的运输条件是什么？
我们的蛋白有液体和冻干粉2种形式，一般来说冻干粉可以用冰袋、蓝冰、干冰运输；液体可以用蓝冰或干冰运输。
Q4:蛋白是通过什么方式纯化的？
一般通过纯化标签进行亲和纯化，常规有镍柱/His Tag、protein AG/Fc tag、标签抗体凝胶等。
Q5:蛋白可以用于细胞培养/注射小鼠吗？
内毒素含量小于0.1 ng/μg (1 EU/μg)的蛋白都可以用于细胞培养/注射小鼠。
Q6:蛋白发货形式可以是冻干粉吗？可以发液体吗？
默认按照说明书标注的形式发货；如果有特殊需求，需要根据蛋白情况确定。原则上，冻干粉蛋白可以发液体形式，液体蛋白不能发冻干粉形式。
Q7:蛋白Buffer里是否含有denaturants（变性剂）？
我们在售重组蛋白一般不含变性剂，具体见说明书的formulation信息。常见变性剂有：尿素和盐酸胍。
Q8:无标签蛋白纯化方式？
蛋白的纯化方式一般通过标签进行亲和纯化，无标签的蛋白是在纯化后进行了标签切除。
Q9:AF蛋白可以提供吗？
在售的重组蛋白都属于Animal component free protein。在售重组蛋白里不含任何直接来源与动物和人的制品：比如血清，血浆，全血，BSA，OVA等。
实验操作视频
新品推荐
Recombinant Mouse Ngfb Protein(His Tag)
Recombinant Mouse IL-18 Protein(Halo Tag)
Recombinant Mouse IL-11 Protein(Sumo Tag)
Recombinant Human VIM Protein(Halo Tag)
Recombinant Rat IL-2 Protein(His Tag)
Recombinant Human GCLC Protein(Trx Tag)
Recombinant Rat IL-17A Protein(hIgG1 Fc Tag)
Recombinant Human IL-17A Protein(Halo Tag)
联系我们
