Recombinant Human Interleukin-13/IL-13 (C-mFc)
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- 表达系统： HEK293 Cells
- 蛋白编码： AAK53823
- 应用性： Cell culture
| 别称 |
BHR;Interleukin;T-Cell Activation Protein P;ALRH;BHR1;T-Cell Activation Protein P600;Interleukin-13;IL13;NC30;P600;Il-13
|
| 表达系统 |
HEK293 Cells
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| 序列 |
Gly21--Asn132
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| 蛋白编码 |
AAK53823
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| 种属 |
Human
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| 计算分子量 |
38.7 kDa
|
| 表观分子量 |
38-60 kDa
|
| 标签 |
C-mFc
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| 生物活性 |
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells The ED50 for this effect is 300 ng/ml.
|
| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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| 制剂 |
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
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| 背景 |
Interleukin-13 is also known as IL-13. It is a protein that in humans is encoded by the IL13 gene. Interleukin-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells.It is involved in several stages of B-cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. This cytokine down-regulates macrophage activity, thereby inhibits the production of pro-inflammatory cytokines and chemokines. This cytokine is found to be critical to the pathogenesis of allergen-induced asthma but operates through mechanisms independent of IgE and eosinophils.
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实验操作视频
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