Recombinant Human Interleukin-17A/IL-17A Protein
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- 表达系统： E.coli
- 蛋白编码： Q16552
- 应用性： Cell culture
| 别称 |
Cytotoxic T-lymphocyte-associated antigen;IL17A;CTLA-8;CTLA8;IL-17;IL-17A;IL17;Interleukin-17A;Cytotoxic T-lymphocyte-associated antigen 8;CTLA 8;CTLA8; CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17; IL-17; IL17A; IL-17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8;Cytotoxic T lymphocyte associated antigen 8;Cytotoxic T lymphocyte associated protein 8;Cytotoxic T lymphocyte associated serine esterase 8;IL 17A;Interleukin 17 (cytotoxic T lymphocyte associated serine esterase 8);Interleukin 17A;Interleukin17;Interleukin17A;OTTHUMP00000016597;OTTMUSP00000046003
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| 表达系统 |
E.coli
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| 序列 |
Ile20-Ala155
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| 蛋白编码 |
Q16552
|
| 种属 |
Human
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| 计算分子量 |
16.5 kDa
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| 表观分子量 |
17 kDa
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| 标签 |
C-His
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| 生物活性 |
Measure by its ability to induce IL-6 secretion in 3T3 cells. The ED50 for this effect is <6 ng/mL.
|
| 纯度 |
> 98 % as determined by reducing SDS-PAGE.
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| 内毒素 |
< 0.01 EU per μg of the protein as determined by the LAL method.
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| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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| 制剂 |
Lyophilized from sterile PBS,pH 8.0.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
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| 背景 |
Interleukin-17 is a potent pro-inflammatory cytokine produced by activated memory T cells. There are at least six members of the IL-17 family in humans and in mice. As IL-17 shares properties with IL-1 and TNF-alpha; it may induce joint inflammation and bone and cartilage destruction. This cytokine is found in synovial fluids of patients with rheumatoid arthritis; and produced by rheumatoid arthritis synovium. It increases IL-6 production; induces collagen degradation and decreases collagen synthesis by synovium and cartilage and proteoglycan synthesis in cartilage. IL-17 is also able to increase bone destruction and reduce its formation. Blocking of interleukin-17 with specific inhibitors provides a protective inhibition of cartilage and bone degradation.
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Q1:重组蛋白有活性吗？可以做活性检测吗？
活性说明的见说明书bio-activity部分；如果说明书显示没有生物活性或则显示生物活性检测中，则说明这个蛋白没有生物活性数据，需要客户自己检测。
Q2:重组蛋白是否测序验证过？
在售重组蛋白默认不做测序分析/验证。
Q3:蛋白的运输条件是什么？
我们的蛋白有液体和冻干粉2种形式，一般来说冻干粉可以用冰袋、蓝冰、干冰运输；液体可以用蓝冰或干冰运输。
Q4:蛋白是通过什么方式纯化的？
一般通过纯化标签进行亲和纯化，常规有镍柱/His Tag、protein AG/Fc tag、标签抗体凝胶等。
Q5:蛋白可以用于细胞培养/注射小鼠吗？
内毒素含量小于0.1 ng/μg (1 EU/μg)的蛋白都可以用于细胞培养/注射小鼠。
Q6:蛋白发货形式可以是冻干粉吗？可以发液体吗？
默认按照说明书标注的形式发货；如果有特殊需求，需要根据蛋白情况确定。原则上，冻干粉蛋白可以发液体形式，液体蛋白不能发冻干粉形式。
Q7:蛋白Buffer里是否含有denaturants（变性剂）？
我们在售重组蛋白一般不含变性剂，具体见说明书的formulation信息。常见变性剂有：尿素和盐酸胍。
Q8:无标签蛋白纯化方式？
蛋白的纯化方式一般通过标签进行亲和纯化，无标签的蛋白是在纯化后进行了标签切除。
Q9:AF蛋白可以提供吗？
在售的重组蛋白都属于Animal component free protein。在售重组蛋白里不含任何直接来源与动物和人的制品：比如血清，血浆，全血，BSA，OVA等。
实验操作视频
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