Recombinant Human Interleukin-2/IL-2 Protein (Mutant)
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- 表达系统： HEK293 Cells
- 蛋白编码： P60568
- 应用性： Cell culture
| 别称 |
IL2;IL-2;TCGF;lymphokine;Aldesleukin;IL-2lymphokine;interleukin-2;Proleukin;interleukin 2;involved in regulation of T-cell clonal expansion;T cell growth factor;T-cell growth factor;IL 2;IL2;Interleukin 2;interleukin2;Involved in regulation of T cell clonal expansion;Lymphokine;POIL2;T Cell Growth Factor;TCGF
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| 表达系统 |
HEK293 Cells
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| 序列 |
Ala21-Thr153(L100F,R101D,L105V,I106V,I112F)
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| 蛋白编码 |
P60568
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| 种属 |
Human
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| 计算分子量 |
16.5 kDa
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| 表观分子量 |
16&18 kDa
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| 标签 |
C-His
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| 生物活性 |
Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. The ED50 for this effect is 151.39 pg/ml.
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| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
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| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
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| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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| 制剂 |
Lyophilized from a 0.2 μm filtered solution of 4mM HCl.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
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| 背景 |
Interleukin-2(IL-2) is an interleukin; a type of cytokine signaling molecule in the immune system;belongs to the IL-2 family. It is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions that are essential for the immune response. IL-2 stimulates growth and differentiation of B-cells; NK cells; lymphokine-activated killer cells; monocytes; macrophages and oligodendrocytes. New research has shown that IL-2 mutant reduced toxicity while being more potent at stimulating anti-tumor effector immune cells.
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实验操作视频
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