Recombinant Human JNK1/MAPK8 Protein (His Tag)
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- 表达系统： E.coli
- 蛋白编码： P45983
| 别称 |
JNK;JNK-46;JNK1;JNK1A2;JNK21B1/2;PRKM8;SAPK1;SAPK1c
|
| 表达系统 |
E.coli
|
| 序列 |
Met1-Glu350
|
| 蛋白编码 |
P45983
|
| 种属 |
Human
|
| 计算分子量 |
38.4 kDa
|
| 表观分子量 |
38 kDa
|
| 标签 |
N-His & C-His
|
| 生物活性 |
Not validated for activity
|
| 纯度 |
> 95% as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 10 EU/mg of the protein as determined by the LAL method
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
|
| 复溶方法 |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
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| 背景 |
Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells. Phosphorylates heat shock factor protein 4.
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实验操作视频
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