Recombinant Human KLK3 protein (His Tag)
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- 表达系统： E.coli
- 蛋白编码： P07288
| 别称 |
APS;Gamma-Seminoprotein;Hk3;KLK2A1;Kallikrein-3;P-30 Antigen;PSA;Prostate-Specific Antigen;Semenogelase;Seminin
|
| 表达系统 |
E.coli
|
| 序列 |
Ile25-Pro261
|
| 蛋白编码 |
P07288
|
| 种属 |
Human
|
| 计算分子量 |
26.0 kDa
|
| 表观分子量 |
32 kDa
|
| 标签 |
N-His
|
| 生物活性 |
Not validated for activity
|
| 纯度 |
> 95% as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 10 EU/mg of the protein as determined by the LAL method
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
|
| 复溶方法 |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
|
| 背景 |
KLK3(Kallikrein 3), also known as Prostate Specific Antigen (PSA), kallikrein-related peptidase 3, Gamma-seminoprotein, is a secreted protein of the glandular kallikrein subfamily of serine proteases.KLK3 contains one peptidase S1 domain.KLK3 is a glycoprotein produced almost exclusively by the prostate gland. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers.
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Q1:重组蛋白有活性吗？可以做活性检测吗？
活性说明的见说明书bio-activity部分；如果说明书显示没有生物活性或则显示生物活性检测中，则说明这个蛋白没有生物活性数据，需要客户自己检测。
Q2:重组蛋白是否测序验证过？
在售重组蛋白默认不做测序分析/验证。
Q3:蛋白的运输条件是什么？
我们的蛋白有液体和冻干粉2种形式，一般来说冻干粉可以用冰袋、蓝冰、干冰运输；液体可以用蓝冰或干冰运输。
Q4:蛋白是通过什么方式纯化的？
一般通过纯化标签进行亲和纯化，常规有镍柱/His Tag、protein AG/Fc tag、标签抗体凝胶等。
Q5:蛋白可以用于细胞培养/注射小鼠吗？
内毒素含量小于0.1 ng/μg (1 EU/μg)的蛋白都可以用于细胞培养/注射小鼠。
Q6:蛋白发货形式可以是冻干粉吗？可以发液体吗？
默认按照说明书标注的形式发货；如果有特殊需求，需要根据蛋白情况确定。原则上，冻干粉蛋白可以发液体形式，液体蛋白不能发冻干粉形式。
Q7:蛋白Buffer里是否含有denaturants（变性剂）？
我们在售重组蛋白一般不含变性剂，具体见说明书的formulation信息。常见变性剂有：尿素和盐酸胍。
Q8:无标签蛋白纯化方式？
蛋白的纯化方式一般通过标签进行亲和纯化，无标签的蛋白是在纯化后进行了标签切除。
Q9:AF蛋白可以提供吗？
在售的重组蛋白都属于Animal component free protein。在售重组蛋白里不含任何直接来源与动物和人的制品：比如血清，血浆，全血，BSA，OVA等。
实验操作视频
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