Recombinant Human LC3A/B Protein(Trx Tag)（PDEH100633）|-Elabscience伊莱瑞特【官网】

Recombinant Human LC3A/B Protein(Trx Tag)

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货号：PDEH100633
说明书

说明书

Recombinant Human LC3A/B Protein(Trx Tag)

Price: ¥3600.00 ¥1200.00

Size:

100 μg 20 μg

表达系统： E.coli
蛋白编码： Q9GZQ8

别称	MAP1A/1BLC;MAP1 light chain 3-like protein;MAP1ALC;MAP1LC3B;ATG8F;LC3B;MAP1A/1BLC3;MAP1LC3B-a;microtubule associated protein 1 light chain 3 beta;Autophagy-related protein LC3 B;Autophagy-related ubiquitin-like modifier LC3 B;LC3A/LC3B;MAP1 light chain 3-like protein 2;MAP1A/MAP1B LC3 B;MAP1A/MAP1B light chain 3 B;Microtubule-associated protein 1 light chain 3 beta;MAP1ALC3;Autophagy related protein LC3 A;Autophagy related ubiquitin like modifier LC3 A;Autophagy related ubiquitin like modifier LC3 B;LC3;LC3II;MAP;MAP1 light chain 3 like protein 1;MAP1 light chain 3 like protein 2;MAP1A/1B light chain 3 A;MAP1LC3B a;Microtubule-associated proteins 1A/1B light chain 3B;MLP3B
表达系统	E.coli
序列	His27-Val125
蛋白编码	Q9GZQ8
种属	Human
计算分子量	30.7 kDa
表观分子量	31 kDa
标签	N-Trx
生物活性	Not validated for activity
纯度	> 90% as determined by reducing SDS-PAGE.
内毒素	< 10 EU/mg of the protein as determined by the LAL method
保存条件	Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
运输条件	This product is provided as lyophilized powder which is shipped with ice packs.
制剂	Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
复溶方法	It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
背景	LC3A, also known as MAP1LC3A, is one of the light chain subunits that function together with both MAP1A and/or MAP1B. MAP1A and MAP1B are microtubule-associated proteins that mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. As a light chain subunit, MAP1LC3A has an important part in neuronal development and in maintaining the balance between neuronal plasticity and rigidity. MAP1LC3A is expressed as two alternatively spliced isoforms that are expressed in testis, brain, heart, liver, and skeletal muscle but are absent in thymus and peripheral blood leukocytes.

SDS-PAGE analysis of Human LC3A/B proteins, 2μg/lane of Recombinant Human LC3A/B proteins was resolved with SDS-PAGE under reducing conditions, showing bands at 31 KD

Q1:重组蛋白有活性吗？可以做活性检测吗？

活性说明的见说明书bio-activity部分；如果说明书显示没有生物活性或则显示生物活性检测中，则说明这个蛋白没有生物活性数据，需要客户自己检测。

Q2:重组蛋白是否测序验证过？

在售重组蛋白默认不做测序分析/验证。

Q3:蛋白的运输条件是什么？

我们的蛋白有液体和冻干粉2种形式，一般来说冻干粉可以用冰袋、蓝冰、干冰运输；液体可以用蓝冰或干冰运输。

Q4:蛋白是通过什么方式纯化的？

一般通过纯化标签进行亲和纯化，常规有镍柱/His Tag、protein AG/Fc tag、标签抗体凝胶等。

Q5:蛋白可以用于细胞培养/注射小鼠吗？

内毒素含量小于0.1 ng/μg (1 EU/μg)的蛋白都可以用于细胞培养/注射小鼠。

Q6:蛋白发货形式可以是冻干粉吗？可以发液体吗？

默认按照说明书标注的形式发货；如果有特殊需求，需要根据蛋白情况确定。原则上，冻干粉蛋白可以发液体形式，液体蛋白不能发冻干粉形式。