Recombinant Human LC3A/B Protein(Trx Tag)
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- 表达系统： E.coli
- 蛋白编码： Q9GZQ8
| 别称 |
MAP1A/1BLC;MAP1 light chain 3-like protein;MAP1ALC;MAP1LC3B;ATG8F;LC3B;MAP1A/1BLC3;MAP1LC3B-a;microtubule associated protein 1 light chain 3 beta;Autophagy-related protein LC3 B;Autophagy-related ubiquitin-like modifier LC3 B;LC3A/LC3B;MAP1 light chain 3-like protein 2;MAP1A/MAP1B LC3 B;MAP1A/MAP1B light chain 3 B;Microtubule-associated protein 1 light chain 3 beta;MAP1ALC3;Autophagy related protein LC3 A;Autophagy related ubiquitin like modifier LC3 A;Autophagy related ubiquitin like modifier LC3 B;LC3;LC3II;MAP;MAP1 light chain 3 like protein 1;MAP1 light chain 3 like protein 2;MAP1A/1B light chain 3 A;MAP1LC3B a;Microtubule-associated proteins 1A/1B light chain 3B;MLP3B
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| 表达系统 |
E.coli
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| 序列 |
His27-Val125
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| 蛋白编码 |
Q9GZQ8
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| 种属 |
Human
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| 计算分子量 |
30.7 kDa
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| 表观分子量 |
31 kDa
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| 标签 |
N-Trx
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| 生物活性 |
Not validated for activity
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| 纯度 |
> 90% as determined by reducing SDS-PAGE.
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| 内毒素 |
< 10 EU/mg of the protein as determined by the LAL method
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| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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| 制剂 |
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
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| 复溶方法 |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
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| 背景 |
LC3A, also known as MAP1LC3A, is one of the light chain subunits that function together with both MAP1A and/or MAP1B. MAP1A and MAP1B are microtubule-associated proteins that mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. As a light chain subunit, MAP1LC3A has an important part in neuronal development and in maintaining the balance between neuronal plasticity and rigidity. MAP1LC3A is expressed as two alternatively spliced isoforms that are expressed in testis, brain, heart, liver, and skeletal muscle but are absent in thymus and peripheral blood leukocytes.
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实验操作视频
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