Recombinant Human LDHA protein (His Tag)
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- 表达系统： E.coli
- 蛋白编码： P00338
| 别称 |
Cell Proliferation-Inducing Gene 19 Protein;L-Lactate Dehydrogenase A Chain;LDH Muscle Subunit;LDH-A;LDH-M;LDHA;Renal Carcinoma Antigen NY-REN-59
|
| 表达系统 |
E.coli
|
| 序列 |
Ala2-Phe332
|
| 蛋白编码 |
P00338
|
| 种属 |
Human
|
| 计算分子量 |
36.3 kDa
|
| 表观分子量 |
39 kDa
|
| 标签 |
N-His
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| 生物活性 |
Not validated for activity
|
| 纯度 |
> 95% as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 10 EU/mg of the protein as determined by the LAL method
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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| 制剂 |
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
|
| 复溶方法 |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
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| 背景 |
L-Lactate Dehydrogenase A Chain (LDHA) is an enzyme that catalyzes the conversion of L-lactate and NAD+ to pyruvate and NADH in the final step of anaerobic glycolysis. LDHA contains an N-terminal coenzyme binding region, a central catalytic site, and at least nine utilized Lys acetylation and two Tyr phosphorylation sites. LDHA belongs to the lactate dehydrogenase family, expressed predominantly in muscle tissue. LDHA mutations have been linked to exertional myoglobinuria.
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实验操作视频
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